Immuno-gold replica technique for ultrastructural localization of antigens
Αποθηκεύτηκε σε:
| Συγγραφείς: | , , |
|---|---|
| Μορφή: | artículo original |
| Κατάσταση: | Versión publicada |
| Ημερομηνία έκδοσης: | 1994 |
| Περιγραφή: | We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid. |
| Χώρα: | Portal de Revistas UCR |
| Ίδρυμα: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Γλώσσα: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/28717 |
| Διαθέσιμο Online: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/28717 |
| Access Level: | acceso abierto |
| Λέξη-Κλειδί : | Sendai virus immunolabelling immunoelectronmicroscopy replica ultrastructure |