In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela

 

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Autores: Buitrago D., Alexis, Rojas-Vera, Janne, Peñaloza, Yonel
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2016
Descripción:Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. In this investigation, phytochemical screening of Vismia baccifera (VB) from Mérida-Venezuela and Vismia macrophylla (VM) from Táchira-Venezuela methanolic extracts, carried out using various chemical assays, revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg mL-1. Phenol and flavonoid assays carried out by Folin-Ciocalteu and colorimetric test also revealed that methanol extracts of both species contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in this investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/22968
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/22968
Access Level:acceso abierto
Palabra clave:antioxidant activity
DPPH
total phenolic content
total flavonoids content
qualitative phytochemical analysis
Vismia baccifera
Vismia macrophylla.
actividad antioxidante
fenoles totales
contenido de flavonoides
análisis fitoquímico cualitativo