Conditioning of mealybug (Hemiptera: Pseudococcidae) by Scanning Electron Microscopy.

 

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Detalles Bibliográficos
Autores: Palma-Jiménez, Melissa, Blanco-Meneses, Mónica
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2015
Descripción:The aim of this work was to determine the methodology for an adequate conditioning for the cleaning of mealybugs specimens and its correct observation. This work was done in the laboratory of the Research Center in Microscopic Structures (CIEMIC) of the University of Costa Rica, in 2012. Four types of methodologies were implemented, which evidenced a gradual improvement of the observation of the ultrastructures through the Scanning Electron Microscopy. Every process was described in detail. The best results were showed with 10% xylene (in some cases it was feasible using 95-100% ethanol). It allowed to remove the wax from the body of the insect, avoiding its collapse, and observing the specific ultrastructures of the individual. This approach will reduce the time and cost of future taxonomic research of mealybugs.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/19330
Acceso en línea:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/19330
Access Level:acceso abierto
Palabra clave:microscopio electrónico de barrido
acondicionamiento de especímenes
ultraestructuras.
scanning electron microscope
specimen conditioning
ultrastructures.