Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
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| Авторы: | , , , , |
|---|---|
| Формат: | artículo original |
| Статус: | Versión publicada |
| Дата публикации: | 2015 |
| Описание: | Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of α-naph- thaleneacetic acid (NAA) and 6-benzylaminopurine (BA). The morphogenetic response obtained of both types of explants, was the formation of protocorm like bodies (PLB’s), that gave place to the formation of new plantlets. Of two tested explants the biggest formation of PLB’s was obtained from the bottom proto- corms sections. The acclimatization of the plantlets was successful, with a 100% percent survival. Plant cell tissue culture represents a useful alternative for the study, conservation and massive propagation of this and other endangered species. |
| Страна: | Portal de Revistas UCR |
| Институт: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Язык: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/19609 |
| Online-ссылка: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19609 |
| Access Level: | acceso abierto |
| Ключевое слово: | micropropagación orquídeas Euchile PLB’s aclimatización conservación ex situ |