Quantification of 17β - estradiol and progesterone hormones in the pink snail Lobatus gigas (Mollusca, Gastropoda)

 

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Autores: Chong Sánchez, Fabiola, Enriquez Diaz, Martha, Aldana Aranda, Dalila
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2019
Descripción:Quantification of steroid hormones in molluscs is performed with different techniques, using the hemolymph or gonads. Lobatus gigas is a Caribbean gastropod of commercial interest indexed in CITES as a protected species. Hormonal studies of this species are as yet unavailable. The objective of this study is to determine the presence of the steroid hormones 17ß - estradiol and progesterone in L. gigas using a non-invasive method, and to compare two techniques for their quantification. Every two months over the course of one year, the feces of ten organisms were collected in Xel-Ha park Quintana Roo, México. The samples were analyzed with High resolution liquid chromatography and Enzyme-linked immunosorbent assays. The values of both hormones were highest during the months of March to September then decreased during November and January. Comparison of the concentrations obtained with HPLC and EIA, presented similar results for 17β-estradiol (Passing - Bablock r = 0.673; mean differences -0.17 ng / ml). In contrast, the progesterone results with both techniques showed no adjustment (Passing - Bablock r = 0.389; mean differences -1.43 ng / ml). Our results suggest that the enzyme-linked immunosorbent assay is suitable for the study of hormones in L. gigas. The knowledge generated will allow the monitoring and selection of breeding organisms that are conditioned in laboratories and thus will not affect the collection of wild egg masses.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/34793
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/34793
Access Level:acceso abierto
Palabra clave:sexual hormones
Lobatus
aquaculture
reproduction
17ß - estradiol
progesterone
Hormonas sexuales
acuacultura
reproducción
progesterona
17ß – estradiol