Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays

 

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Detalles Bibliográficos
Autores: Chaves, Fernando, León, Guillermo, Hernández Chavarría, Francisco
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2006
Descripción:Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/14108
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108
Access Level:acceso abierto
Palabra clave:clostridium tetani
tetanus toxin
enzyme immunoassay
biological assay