Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

 

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
লেখক: Solís-Ramos, Laura-Yesenia, Ortiz-Pavón, Julio-César, Andrade-Torres, Antonio, Porras-Murillo, Romano, Brenes-Angulo, Arturo, Castaño de la Serna, Enrique
বিন্যাস: artículo original
বর্তমান অবস্থা:Versión publicada
প্রকাশনার তারিখ:2019
বিবরন:Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.
দেশ:Portal de Revistas UCR
প্রতিষ্ঠান:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
ভাষা:Inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/37208
অনলাইন ব্যবহার করুন:https://revistas.ucr.ac.cr/index.php/rbt/article/view/37208
Access Level:acceso abierto
মুখ্য শব্দ:common bean; recalcitrant species; genetic transformation; A. tumefaciens; gusA activit; nptII
frijol común; especies recalcitrantes; transformación genética; A. tumefaciens; actividad gusA; nptII