Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca
সংরক্ষণ করুন:
লেখক: | , , , , , |
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বিন্যাস: | artículo original |
বর্তমান অবস্থা: | Versión publicada |
প্রকাশনার তারিখ: | 2019 |
বিবরন: | Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene. |
দেশ: | Portal de Revistas UCR |
প্রতিষ্ঠান: | Universidad de Costa Rica |
Repositorio: | Portal de Revistas UCR |
ভাষা: | Inglés |
OAI Identifier: | oai:portal.ucr.ac.cr:article/37208 |
অনলাইন ব্যবহার করুন: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/37208 |
Access Level: | acceso abierto |
মুখ্য শব্দ: | common bean; recalcitrant species; genetic transformation; A. tumefaciens; gusA activit; nptII frijol común; especies recalcitrantes; transformación genética; A. tumefaciens; actividad gusA; nptII |