Effect of BAP and IAA on shoot regeneration in cotyledonary explants of Costa Rican melon genotypes
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| Autores: | , |
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| 格式: | artículo original |
| 状态: | Versión publicada |
| Fecha de Publicación: | 2008 |
| 实物特征: | Cultured cotyledon explants of OSO-1, OSO-2, OSO-3, PQRG-1, PQRG-2, PQRG-3, and EM-1 "criollo" melon (Cucumis melo L) genotypes were evaluated with regard to their morphogenic response to combinations of N6-benzylaminopurine (BAP) (0.1, 0.5 and 1.0 mg.l-1) with indolacetic acid (IAA) (0, 0.05 and 0.5 mg.l-1). Regardless of BAP and IAA concentration in the shoot induction medium, the highest shoot formation percentages were obtained using EM-1 > OSO-1 > PQRG-3 > OSO- 2 > PQRG-2 > PQRG-1 > OSO-3. On the other hand, independently of the genotype, the shoot induction medium supplemented with 0.5 mg.l-1 BAP and 0.05 mg.l-1 IAA or 1 mg.l-1 BAP and 0 IAA mg.l-1 resulted in the highest average of shoots. Culture of cotyledons of the genotypes evaluated on induction medium supplemented with different BAP and IAA resulted in a different response. The in vitro culture protocol developed in this study will be useful in micropropagation of "criollo" melon genotypes. |
| País: | Portal de Revistas UCR |
| 机构: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| 语言: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/6740 |
| 在线阅读: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/6740 |
| Access Level: | acceso abierto |
| Palabra clave: | Melón "criollo" Cucumis melo L regeneración de plantas cultivo in vitro morfogénesis "Criollo" melon plant regeneration in vitro culture morphogenesis |