Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica

 

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Detalles Bibliográficos
Autores: Vásquez-Mayorga, Marcela, Fuchs, Eric, Hernández, Eduardo, Herrera, Franklin, Hernández, Jesús, Moreira-González, Ileana, Arnáez-Serrano, Elizabeth, Barboza, Natalia
Formato: artículo original
Fecha de Publicación:2017
Descripción:We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor- Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He D 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC D 0.274) and inbreeding coefficient (f D0:102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica.
País:RepositorioTEC
Institución:Instituto Tecnológico de Costa Rica
Repositorio:RepositorioTEC
Lenguaje:Español
OAI Identifier:oai:repositoriotec.tec.ac.cr:2238/7095
Acceso en línea:https://peerj.com/articles/2931/
https://hdl.handle.net/2238/7095
Access Level:acceso abierto
Palabra clave:Diversidad genética
Filogenética
Toxicidad
Jatropha curcas
Genes
Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Molecular biology
Marcadores -- EST-SSR -- G-SSR -- nrDNA-ITS
Genetic diversity
Phylogenetics
Toxicity
Markers -- EST-SSR -- G-SSR -- nrDNA-ITS
Marcadores G-SSR -- SCAR
Markers -- G-SSR -- SCAR