Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica
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Autores: | , , , , , , , |
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Formato: | artículo original |
Fecha de Publicación: | 2017 |
Descripción: | We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor- Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He D 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC D 0.274) and inbreeding coefficient (f D0:102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica. |
País: | RepositorioTEC |
Institución: | Instituto Tecnológico de Costa Rica |
Repositorio: | RepositorioTEC |
Lenguaje: | Español |
OAI Identifier: | oai:repositoriotec.tec.ac.cr:2238/7095 |
Acceso en línea: | https://peerj.com/articles/2931/ https://hdl.handle.net/2238/7095 |
Access Level: | acceso abierto |
Palabra clave: | Diversidad genética Filogenética Toxicidad Jatropha curcas Genes Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Molecular biology Marcadores -- EST-SSR -- G-SSR -- nrDNA-ITS Genetic diversity Phylogenetics Toxicity Markers -- EST-SSR -- G-SSR -- nrDNA-ITS Marcadores G-SSR -- SCAR Markers -- G-SSR -- SCAR |