Cryopreservation of chayote (Sechium edule JACQ.SW.) zygotic embryos and shoot-tips from in vitroplantlets

 

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Detalles Bibliográficos
Autores: Abdelnour-Esquivel, Ana, Engelmann, Florent
Formato: artículo original
Fecha de Publicación:2002
Descripción:This paper presents the development of cryopreservation protocols for zygotic embryos and apices of chayote (Sechium edule Jacq. Sw.), a tropical plant species with recalcitrant seeds. Zygotic embryos of two cultivars, Ccocro negro (CN) and Claudio (Cl) could withstand cryopreservation, with survival percentages of 10 and 30 %, after desiccation to 23 and 19 % moisture content (fresh weight basis), respectively. Apices sampled on in vitro plantlets of cultivars Cl, 13 and JM were successfully cryopreserved using a vitrification technique. Optimal conditions included the culture of mother-plants for 22 days on medium containing 0.3 M sucrose, culture of excised apices on the same medium for 1 day, loading of apices for 20 min with 2M glycerol + 0.4M glycerol, treatment with a series of diluted PVS2 solution (60 % PVS2 followed by 80 % PVS2 solution for 15 min (cultivar Cocoro Blanco [CB]) or 30 min (cultivars CN and Cl) at each concentration), rapid freezing and thawing, washing of shoot-tips with a 1.2 M sucrose solution, followed by recovery on media with progressively decreasing sucrose concentrations until the standard concentration of 0.1 M was reached. The highest survival percentages achieved ranged between 17 and 38 %, depending on the cultivar
País:RepositorioTEC
Institución:Instituto Tecnológico de Costa Rica
Repositorio:RepositorioTEC
Lenguaje:Inglés
OAI Identifier:oai:repositoriotec.tec.ac.cr:2238/6951
Acceso en línea:https://www.ingentaconnect.com/content/cryo/cryo/2002/00000023/00000005/art00004
https://hdl.handle.net/2238/6951
Access Level:acceso abierto
Palabra clave:Chayote
Sechium edule
Criopreservación
Embriones
Desecación
Sacarosa
In vitro
Cryopreservation
Embryos
Desiccation
Sucrose