Micropropagación de chayote (Sechium edule) a partir de brotes vegetativos

 

Authors
Ramírez, Carlos; Engelmann, Florent; Abdelnour-Esquivel, Ana
Format
Article
Status
publishedVersion
Description

Micropropagation of waterpear (Sechium edule Jacq. S.W.) from vegetative shoots. Waterpear (Sechium edule) is an important source of revenue for the exporting countries, being Costa Rica the leading producer. Among the main limiting factors for its production are the planting materials, traditionally the seed, that because of its nature it does not allow the production of morphologically homogeneous fruits (color, shape and epidermal texture), which results in high percentages of rejected fruits at the packing plant. The establishment of a protocol for vegetative propagation of this species will help to solve the heterogeneity problems. The present research was directed to develop a micropropagation protocol from shoots. The effect of various desinfectants and concentration of growth regulators during the in vitro establishment, multiplication and rooting phases were evaluated. Desinfections with 4% calcium hypochlorite allowed the highest percentage of aseptically established explants under in vitro conditions. There was also observed that during the following phases of micropropagation the requirements of growth regulators were very low (0.1 mgL-1 BA during bud sprouting and 0.1 to 0.2 mgL-1 AIB during rooting) or were not required (microcutting multiplication).

Publication Year
2002
Language
spa
Topic
Siembra
Rechazo
Micropropagación
Hipoclorito de calcio
Chayote
Fuente
RepositorioTEC
Get full text
http://hdl.handle.net/2238/6952
Derechos
openAccess
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