Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica
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| Autoren: | , , |
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| Format: | artículo |
| Publikationsdatum: | 2015 |
| Beschreibung: | A competitive enzyme-linked immunosorbent assay (c-ELISA) was compared to the serum neutralization test (SNT) for the detection of antibodies against vesicular stomatitis virus, New Jersey (VSV-NJ) and Indiana (VSV-IN) serotypes, using 214 equine serum samples from Costa Rica. In the c-ELISA for VSV-NJ 109 (50.93%) equine sera reacted positive, whereas in the SNT 138 (64.48%) serum samples reacted positive to VSV-NJ. In the c-ELISA for VSV-IN 26 (12.15%) sera reacted positive, whereas in the SNT 36 (16.83%) equine sera reacted positive to VSV-IN. The c-ELISA showed a moderate sensitivity compared to the SNT. The rapidity and ease of the c-ELISA system makes it suitable as a rapid diagnostic screening assay. However, because of the lower sensitivity of the c-ELISA, the SNT is considered the method of choice to detect equine VSV infections in Costa Rica. |
| Land: | Repositorio UNA |
| Institution: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Sprache: | Inglés |
| OAI Identifier: | oai:https://repositorio.una.ac.cr:11056/17804 |
| Online Zugang: | http://hdl.handle.net/11056/17804 |
| Access Level: | acceso abierto |
| Stichwort: | ESTOMATITIS CABALLOS VIRUS ELISA MEDICINA VETERINARIA COSTA RICA VESICULAR STOMATITIS VIRUS SEROLOGY EQUINE DIAGNOSIS |