Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo)

 

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Detalles Bibliográficos
Autores: Lomonte, Bruno, Gutiérrez, José María, Moreno Robles, Edgardo, Cerdas Fallas, Luis
Formato: artículo original
Fecha de Publicación:1987
Descripción:Neutralization of biological activities of B. asper myotoxin by a monospecific anti-myotoxin rabbit serum and polyvalent antivenom was studied. Both antisera neutralized myotoxic, phospholipase A and lethal activities of myotoxin, whereas edema-forming activity of myotoxin was not neutralized by these antisera. Anti-myotoxin rabbit serum neutralized most of the myotoxic activity of B. asper venom, but did not neutralize phospholipase A activity of this venom. Neutralization curves showed that myotoxicity induced by myotoxin persisted at antivenom/toxin or antiserum/toxin ratios at which phospholipase A activity was completely neutralized. This suggests that myotoxicity does not depend upon enzymatic activity of the toxin. Antiserum to myotoxin neutralized approximately 70% of myonecrosis induced by crude B. asper venom. This demonstrates that myotoxin is the main factor responsible for the development of myonecrosis in envenomations by B. asper.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/29148
Acceso en línea:http://www.sciencedirect.com/science/article/pii/004101018790078X
https://hdl.handle.net/10669/29148
Access Level:acceso abierto
Palabra clave:Animals
Antivenins
Crotalid Venoms
Muscles
Necrosis
Neutralization Tests
Phospholipases A
Snake venom