Molecular Detection of Bartonella Species in Fleas Collected from Dogs and Cats from Costa Rica

 

Guardado en:
Detalles Bibliográficos
Autores: Rojas Campos, Norman, Troyo Rodríguez, Adriana, Castillo, Daniela, Gutiérrez, Ricardo, Harrus, Shimon
Formato: artículo original
Fecha de Publicación:2015
Descripción:The bacterial genus Bartonella includes several species with zoonotic potential, some of which are common in domestic dogs and cats, as well as in their fleas. Because there is no previous information about the presence of Bartonella species in fleas from Central America, this study aimed at evaluating the presence of Bartonella spp. in fleas collected from dogs and cats in Costa Rica. A total 72 pools of Ctenocephalides felis and 21 pools of Pulex simulans were screened by conventional PCR to detect Bartonella DNA fragments of the citrate synthase (gltA) and the b subunit RNA polymerase (rpoB) genes. Three (4.2%) pools of C. felis and five pools (22.7%) of P. simulans were found positive for Bartonella DNA. Sequences corresponding to Bartonella vinsonii subsp. berkhoffii strain Winnie, B. rochalimae, and an undescribed Bartonella sp. (clone BR10) were detected in flea pools from dogs, whereas Bartonella henselae and B. clarridgeiae sequences were identified in flea pools from cats. The detection of zoonotic Bartonella spp. in this study should increase the awareness to these flea borne diseases among physicians and public health workers and highlight the importance of flea control in the region.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/74857
Acceso en línea:https://www.liebertpub.com/doi/10.1089/vbz.2015.1799
https://hdl.handle.net/10669/74857
Access Level:acceso abierto
Palabra clave:Bartonella
Bartonellosis
Ctenocephalides felis
Pulex
Dogs
Cats
Domestic animals
PCR
595. 775 728 6 Molecular Detection of Bartonella Species in Fleas Collected from Dogs and Cats from Costa Rica