Impact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches

 

Guardado en:
Detalles Bibliográficos
Autores: Gutiérrez, José María, Sanz, Libia, Flores Díaz, Marietta, Figueroa, Lucía, Madrigal Villalobos, Marvin, Herrera Vega, María, Villalta Arrieta, Mauren, León Montero, Guillermo, Estrada Umaña, Ricardo, Borges, Adolfo, Alape Girón, Alberto, Calvete Chornet, Juan José
Formato: artículo original
Fecha de Publicación:2010
Descripción:Intraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was assessed by comparing two commercial and four experimental antivenoms. All antivenoms showed similar immunorecognition pattern toward the venoms from adult and neonate specimens. They completely immunodepleted most P−III snake venom metalloproteinases (SVMPs), l-amino acid oxidases, serine proteinases, DC fragments, cysteine-rich secretory proteins (CRISPs), and C-type lectin-like proteins, and partially immunodepleted medium-sized disintegrins, phospholipases A2 (PLA2s), some serine proteinases, and P−I SVMPs. Although all antivenoms abrogated the lethal, hemorrhagic, coagulant, proteinase, and PLA2 venoms activities, monospecific experimental antivenoms were more effective than the polyspecific experimental antivenom. In addition, the commercial antivenoms, produced in horses subjected to repeated immunization cycles, showed higher neutralization than experimental polyspecific antivenom, produced by a single round of immunization. Overall, a conspicuous pattern of cross-neutralization was evident for all effects by all antivenoms, and monospecific antivenoms raised against venom from the Caribbean population were effective against venom from the Pacific population, indicating that geographic variations in venom proteomes of B. asper from Costa Rica do not result in overt variations in immunological cross-reactivity between antivenoms.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/29348
Acceso en línea:http://pubs.acs.org/doi/abs/10.1021/pr9009518
https://hdl.handle.net/10669/29348
Access Level:acceso abierto
Palabra clave:Antivenom
Antivenomics
Bothrops asper
Phospholipases A2
Snake venom metalloproteinase
Snake venom proteome
Venom neutralization assays
Venomics
Snake venom