Effect of Bothrops asper (Fer-De-Lance) snake venom on erythrocyte membrane. A comparative study
Guardado en:
| Autores: | , , , |
|---|---|
| Formato: | artículo original |
| Fecha de Publicación: | 1992 |
| Descripción: | 1. The effect of Bothrops asper venom on erythrocytes of different species, sheep erythrocyte ghost vesicles and liposomes made of phospholipids from sheep erythrocytes was studied. 2. B. asper venom had a direct hemolytic effect on mouse erythrocytes, but no lytic activity on goat, rabbit, horse, toad and sheep erythrocytes. Human erythrocytes were lysed only in the presence of bovine serum albumin and Ca2+. 3. Although the phospholipase A2 activity in the venom might be involved in the lytic effect on mouse erythrocytes, there is also evidence of direct lytic factor(s) acting in the absence of calcium ions. 4. Sheep erythrocytes were modified in order to study the basis of their resistance. Enhancement of membrane fluidity and variation of pH were of no consequence for the resistance of sheep erythrocytes to the action of venom. 5. The reorganization of membranes that takes place during ghost or liposome preparation made them susceptible to B. asper venom-induced disruption. Thus the resistance of sheep erythrocytes seems related to the accessibility of the target. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:https://www.kerwa.ucr.ac.cr:10669/29174 |
| Acceso en línea: | https://hdl.handle.net/10669/29174 |
| Access Level: | acceso abierto |
| Palabra clave: | Animals Anura Calcium Crotalid Venoms Erythrocyte Membrane Goats Hemolysis Horses Humans Hydrogen-Ion Concentration Liposomes Membrane Fluidity Mice Rabbits Serum Albumin, Bovine Sheep Species Specificity |