Variation in the whole mitogenome of reef-building Porites corals

 

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Detalles Bibliográficos
Autores: Paz García, David A., Galván Tirado, Carolina, Alvarado Barrientos, Juan José, Cortés Núñez, Jorge, García de León, Francisco Javier, Hellberg, Michael E., Balart, Eduardo F.
Formato: artículo original
Fecha de Publicación:2016
Descripción:The study of complete mitochondrial genomes (mitogenomes) revealed different gene rearrangements, highly variable markers, and delineated clades that have aided the understanding of the evolutionary history in corals. In this study, we examined mitogenomic variation of reef-building Porites corals and designed 34 primer pairs to target high diversity regions and to amplify the complete mitogenome of a widely-distributed Indo-Pacific species of Porites (P. lobata) and two endemic species of the Eastern Pacific (P. sverdrupi and P. panamensis). All primer pairs amplified for each species and the mitogenomes assembled yielded the same gene order as obtained from next-generation sequencing. Mitogenomic variation in Porites corals was three to ten times higher than in Acropora or Pocillopora, two other major reef builders. In contrast to these corals, the nucleotide variation in Porites species was distributed evenly along the mitogenome. Primers designed here are useful to amplify regions with the highest variation possible as well as to assemble the whole mitogenomes of different Porites species. The resulting mitogenomes should improve our understanding of evolutionary relationships, delimitation of species, and conservation within the genus Porites.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/73434
Acceso en línea:https://link.springer.com/article/10.1007%2Fs12686-016-0527-x
https://hdl.handle.net/10669/73434
Access Level:acceso abierto
Palabra clave:Mitochondrial genome
Porites corals
Eastern Pacific
Scleractinian corals