Streptomyces sp. M54: an actinobacteria associated with a neotropical social wasp with high potential for antibiotic production

 

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Detalles Bibliográficos
Autores: Matarrita Carranza, Bernal, Murillo Cruz, Catalina, Avendaño Vega, Roberto, Ríos Marín, María Isabel, Chavarría Vargas, Max, Gómez Calvo, María Luisa, Tamayo Castillo, Giselle, Araya Barrantes, Juan José, Pinto Tomás, Adrián A.
Formato: artículo original
Fecha de Publicación:2021
Descripción:Abstract Streptomyces symbionts in insects have shown to be a valuable source of new antibiotics. Here, we report the genome sequence and the potential for antibiotic production of ‘‘Streptomyces sp. M54’’, an Actinobacteria associated with the eusocial wasp, Polybia plebeja. The Streptomyces sp. M54 genome is composed of a chromosome (7.96 Mb), and a plasmid (1.91 Kb) and harbors 30 biosynthetic gene clusters for secondary metabolites, of which only one third has been previously characterized. Growth inhibition bioassays show that this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens taphylococcus aureus and Candida albicans. Analyses through TLC-bioautography, LC–MS/MS and NMR allowed the identification of five macrocyclic ionophore antibiotics, with previously reported antibacterial, antitumor and antiviral properties. Phylogenetic analyses placed Streptomyces sp.M54 in a clade of other host-associated strains taxonomically related to Streptomyces griseus. Pangenomic and ANI analyses confirm the identity of one of its closest relatives as Streptomyces sp. LaPpAH-199, a strain isolated from an ant-plant symbiosis in Africa. In summary, our results suggest an insect-microbe association in distant geographic areas and showcase the potential of Streptomyces sp. M54 and related strains for the discovery of novel antibiotics
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
Lenguaje:Inglés
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/83215
Acceso en línea:https://hdl.handle.net/10669/83215
Access Level:acceso abierto
Palabra clave:Streptomyces
Polybia
Symbiosis
Nonactin
Macrotetrolide antibiotics
Genome mining