Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities

 

Guardado en:
Detalles Bibliográficos
Autores: Antúnez Oliva, José, Fernández Ulate, Julián, Lomonte, Bruno, Angulo Ugalde, Yamileth, Sanz, Libia, Pérez, Alicia, Calvete Chornet, Juan José, Gutiérrez, José María
Formato: artículo original
Fecha de Publicación:2010
Descripción:Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an ‘antivenomics’ approach, the ability of a polyspecific (polyvalent) antivenom manufactured in Costa Rica to recognize the proteins of Atropoides mexicanus and A. picadoi venoms, which are not included in the immunization mixture. In addition, the neutralization of lethal, hemorrhagic, myotoxic, coagulant, proteinase and phospholipase A2 (PLA2) activities of these venoms by the antivenom was assessed. The antivenom was highly-effective in immunodepleting many venom components, particularly high molecular mass P-III metalloproteinases (SVMPs), L-amino acid oxidases, and some serine proteinases and P-I SVMPs. In contrast, PLA2s, certain serine proteinases and P-I SVMPs, and a C type lectin-like protein were only partially immunodepleted, and two PLA2 molecules were not depleted at all. The antivenom was able to neutralize all toxic and enzymatic activities tested, although neutralization of lethality by A. nummifer venom was achieved when a challenge dose of 3 LD50s of venom was used, but was iffective when 4 LD50s were used. These results, and previously obtained evidence on the immunoreactivity of this antivenom towards homologous and heterologous venoms, revealed the low immunogenicity of a number of venom components (PLA2s, CRISPs, P-I SVMPs, and some serine proteinases), underscoring the need to search for innovative immunization protocols to improve the immune response to these antigens.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/29318
Acceso en línea:http://libpubmedia.co.uk/antivenomics-of-atropoides-mexicanus-and-atropoides-picadoi-snake-venoms-relationship-to-the-neutralization-of-toxic-and-enzymatic-activities/
https://hdl.handle.net/10669/29318
Access Level:acceso abierto
Palabra clave:Atropoides picadoi
Atropoides mexicanus
Antivenomics
Antivenom
Neutralization
Toxicity
Immunodepletion
Snake venom