Brucella genetic variability in wildlife marine mammals populations relates to host preference and ocean distribution

 

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Detalles Bibliográficos
Autores: Suárez-Esquivel, Marcela, Baker, Kate, Ruiz-Villalobos, Nazareth, Hernandez-Mora, Gabriela, BARQUERO-CALVO, ELIAS, González-Barrientos, Rocío, Castillo-Zeledón, Amanda, Jiménez-Rojas, César, Chacón-Díaz, Carlos, Cloeckaert, Axel, Chaves-Olarte, Esteban, Thomson, Nicholas, Moreno, Edgardo, Guzman-Verri, Caterina
Formato: artículo
Fecha de Publicación:2017
Descripción:Intracellular bacterial pathogens probably arose when their ancestor adapted from a free-living environment to an intracellular one, leading to clonal bacteria with smaller genomes and less sources of genetic plasticity. Still, this plasticity is needed to respond to the challenges posed by the host. Members of the Brucella genus are facultative-extracellularintracellularbacteriaresponsibleforcausing brucellosis in a variety of mammals. The various species keep different host preferences, virulence, and zoonotic potential despite having 97–99% similarity at genome level. Here, we describe elements of genetic variation in Brucella ceti isolated from wildlife dolphins inhabiting the Pacific Ocean and the Mediterranean Sea. Comparison with isolates obtained from marine mammals from the Atlantic Ocean and the broader Brucella genus showed distinctive traits according to oceanic distribution and preferred host. Marine mammal isolates display genetic variability, represented by an important number of IS711 elements as well as specific IS711 and SNPs genomic distribution clustering patterns. Extensive pseudogenization was found among isolates from marine mammals as compared with terrestrial ones, causing degradation in pathways related to energy, transport of metabolites, and regulation/transcription. Brucella ceti isolates infecting particularly dolphin hosts, showed further degradation of metabolite transport pathways as well as pathways related to cell wall/membrane/envelope biogenesis and motility. Thus, gene loss through pseudogenization is a source of genetic variation in Brucella, which in turn, relates to adaptation to different hosts. This is relevant to understand the natural history of bacterial diseases, their zoonotic potential, and the impact of human interventions such as domestication.
País:Repositorio UNA
Institución:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
Lenguaje:Inglés
OAI Identifier:oai:https://repositorio.una.ac.cr:11056/18352
Acceso en línea:http://hdl.handle.net/11056/18352
Access Level:acceso abierto
Palabra clave:BRUCELLA
BRUCELOSIS
MAMIFEROS MARINOS
MARINE MAMMALS
GENOME DEGRADATION
GENOTIPOS