Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum

 

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
লেখক: Celli, Jean, Chastellier, Chantal de, Franchini, Don Marc, Pizarro Cerda, Javier, Gorvel, Jean Pierre, Moreno, Edgardo
বিন্যাস: artículo
প্রকাশনার তারিখ:2003
বিবরন:The intracellular pathogen Brucella is the causative agent of brucellosis, a worldwide zoonosis that affects mammals, including humans. Essential to Brucella virulence is its ability to survive and replicate inside host macrophages, yet the underlying mechanisms and the nature of the replica tive compartment remain unclear. Here we show in a model of Brucella abortus infection of mu rine bone marrow–derived macrophages that a fraction of the bacteria that survive an initial macrophage killing proceed to replicate in a compartment segregated from the endocytic path way. The maturation of the Brucella-containing vacuole involves sustained interactions and fusion with the endoplasmic reticulum (ER), which creates a replicative compartment with ER-like properties. The acquisition of ER membranes by replicating Brucella is independent of ER-Golgi COPI-dependent vesicular transport. A mutant of the VirB type IV secretion system, which is necessary for intracellular survival, was unable to sustain interactions and fuse with the ER, and was killed via eventual fusion with lysosomes. Thus, we demonstrate that live intracellular Bru cella evade macrophage killing through VirB-dependent sustained interactions with the ER. Moreover, we assign an intracellular function to the VirB system, as being required for late mat uration events necessary for the biogenesis of an ER-derived replicative organelle.
দেশ:Repositorio UNA
প্রতিষ্ঠান:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
ভাষা:Inglés
OAI Identifier:oai:https://repositorio.una.ac.cr:11056/22950
অনলাইন ব্যবহার করুন:http://hdl.handle.net/11056/22950
https://doi.org/10.1084/jem.20030088
Access Level:acceso abierto
মুখ্য শব্দ:BRUCELOSIS
BRUCELLA
BRUCELLA ABORTUS
MAMIFEROS
MAMMALS
TYPE IV SECRETION