Brucella abortus senses the intracellular environment through the BvrR/Bvrs two-component system, which allows B. abortus to adapt to its replicative nich

 

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Autores: Altamirano-Silva, Pamela, Meza-Torres, Jazmín, Castillo-Zeledón, Amanda, Ruiz-Villalobos, Nazareth, Zúñiga Pereira, Ana Mariel, Chacón-Díaz, Carlos, Moreno, Edgardo, Guzman-Verri, Caterina, Chaves-Olarte, Esteban
格式: artículo
Fecha de Publicación:2018
实物特征:Brucella abortus is a facultative extracellular-intracellular pathogen belonging to a group of Alphaproteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in a membrane-bound compartment, avoiding the lysosomal route and reaching the endoplasmic reticulum through the action of the type IV secretion system, VirB. In this work, we demonstrate that the BvrR/BvrS two-component system senses the intracellular environment to mount the transcriptional response required for intracellular life adaptation. By combining a method to purify intracellularly extracted bacteria with a strategy that allows direct determination of BvrR phosphorylation, we showed that upon entrance to host cells, the regulatory protein BvrR was activated (BvrR-P) by phosphorylation at aspartate 58. This activation takes place in response to intracellular cues found in early compartments, such as low pH and nutrient deprivation. Furthermore, BvrR activation was followed by an increase in the expression of VjbR and VirB. The in vitro activation of this BvrR-P/VjbR/VirB virulence circuit rescued B. abortus from the inhibition of intracellular replication induced by bafilomycin treatment of cells, demonstrating the relevance of this mechanism for intracellular bacterial survival and replication. All together, our results indicate that B. abortus senses the transition from the extracellular to the intracellular milieu through BvrR/BvrS, allowing the bacterium to transit safely to its replicative niche. These results serve as a working model for understanding the role of this family of two-component systems in the adaptation to intracellular life of Alphaproteobacteria
País:Repositorio UNA
机构:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
语言:Inglés
OAI Identifier:oai:https://repositorio.una.ac.cr:11056/18332
在线阅读:http://hdl.handle.net/11056/18332
https://doi.org/10.1128/IAI.00713-17.
Access Level:acceso abierto
Palabra clave:BRUCELOSIS
BRUCELLA
TYPE IV SECRETION SYSTEM
BACTERIAS
BRUCELLA ABORTUS