Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC–QqTOF-MS
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| Autores: | , , , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 2011 |
| Descripción: | Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L−1 . Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC–QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N4 -formyl-SMZ, N4 -hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-13C6 after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfon amides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/89943 |
| Acceso en línea: | https://www-sciencedirect-com.ezproxy.sibdi.ucr.ac.cr/science/article/pii/S0048969711008862 https://hdl.handle.net/10669/89943 |
| Access Level: | acceso abierto |
| Palabra clave: | Sulfamethazine Trametes versicolor Sewage sludge Degradation metabolites UPLC–QqTOF-MS analysis |