Molecular identification of Trichoderma spp. in garlic and onion fields and In Vitro antagonism trials on Sclerotium cepivorum

 

Authors
Alvarado-Marchena, Luis; Rivera-Méndez, William
Format
Article
Status
publishedVersion
Description

Artículo científico
Trichoderma species are non-pathogenic microorganisms that protect against fungal diseases and contribute to increased crop yields. However, not all Trichoderma species have the same effects on crop or a pathogen, whereby the characterization and identification of strains at the species level is the first step in the use of a microorganism. The aim of this study was the identification – at species level – of five strains of Trichoderma isolated from soil samples obtained from garlic and onion fields located in Costa Rica, through the analysis of the ITS1, 5.8S, and ITS2 ribosomal RNA regions; as well as the determination of their individual antagonistic ability over S. cepivorum Berkeley. In order to distinguish the strains, the amplified products were analyzed using MEGA v6.0 software, calculating the genetic distances through the Tamura-Nei model and building the phylogenetic tree using the Maximum Likelihood method. We established that the evaluated strains belonged to the species T. harzianum and T. asperellum; however it was not possible to identify one of the analyzed strains based on the species criterion. To evaluate their antagonistic ability, the dual culture technique, Bell’s scale, and the percentage inhibition of radial growth (PIRG) were used, evidencing that one of the T. asperellum isolates presented the best yields under standard, solid fermentation conditions.

Publication Year
2016
Language
Inglés
Topic
Ribosomas
Análisis
Filogenética
Microorganismos
Patógenos
Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology
Fuente
RepositorioTEC
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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832016000100404
http://hdl.handle.net/2238/9810
Derechos
openAccess
Licencia
Attribution-NonCommercial-ShareAlike 4.0 International