Assessment of the impact of solvent/detergent treatment on the quality and potency of a whole IgG equine antivenom
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| Autoren: | , , , , |
|---|---|
| Format: | artículo original |
| Publikationsdatum: | 2009 |
| Beschreibung: | We have evaluated for the first time the impact of a solvent/detergent (S/D) treatment on the quality and in vivo neutralization potency of horse-derived whole IgG antivenom used in the treatment of viperid snake bite envenoming in Central America. The S/D treatment by 1% tri (n-butyl) phosphate (TnBP) – 1% Triton X-45 at 22–25 °C was applied either on starting plasma or on purified immunoglobulins. The S/D agents were removed from both fractions by extractions with oil. S/D-treated plasma was subjected to caprylic acid precipitation to purify the immunoglobulins. Products were formulated, sterile-filtered, and filled into 10-mL vials, stored at 5 ± 3 °C, and subjected to routine quality controls, SDS-PAGE, determination of anti-Bothrops asper venom antibody titre by ELISA, in vivo B. asper venom-neutralization potency tests, and safety test, comparatively with an antivenom manufactured by caprylic acid fractionation without S/D treatment. Results indicate that these conditions of S/D treatment on purified immunoglobulin yielded an antivenom of high turbidity that induced weight loss in animals. In contrast, antivenom fractionated from the S/D-treated plasma had physico-chemical and biological characteristics indistinguishable from those of the non-S/D-treated antivenom. S/D treatment of horse plasma may be considered to increase the viral safety of antivenoms. |
| Land: | Kérwá |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Sprache: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104303 |
| Online Zugang: | https://hdl.handle.net/10669/104303 https://doi.org/10.1016/j.biologicals.2009.05.003 |
| Stichwort: | antivenom snake bite envenoming viral inactivation solvent/detergent neutralization |