Snake venom metalloproteinases: structure/function relationships studies using monoclonal antibodies
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| Auteurs: | , , , , , , , , |
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| Formaat: | artículo original |
| Publicatiedatum: | 2003 |
| Omschrijving: | Snake Venom Metalloproteinases (SVMPs) are synthesized as zymogens and undergo proteolytic processing resulting in a variety of multifunctional proteins. Jararhagin is a P-III SVMP, isolated from the venom of Bothrops jararaca, comprising metalloproteinase, disintegrin-like and cysteine-rich domains. The catalytic domain is responsible for the hemorrhagic activity. The disintegrin-like/cysteine-rich domains block α2β1 integrin binding to collagen and apparently enhance the hemorrhagic activity of SVMPs. The relevance of disintegrin-like domain is described in this paper using a series of mouse anti-jararhagin monoclonal antibodies (MAJar 1–7). MAJar 3 was the only antibody able to completely neutralize jararhagin hemorrhagic activity. Neutralization of catalytic activity was partial by incubation with MAJar 1. MAJars 1 and 3 efficiently neutralized jararhagin binding to collagen with IC50 of 330 and 8.4 nM, respectively. MAJars 1 and 3 recognized the C-terminal portion of the disintegrin domain, which is apparently in conformational proximity with the catalytic domain according to additivity tests. These data suggest that disintegrin-like domain epitopes are in close contact with catalytic site or functionally modulate the expression of hemorrhagic activity in SVMPs. |
| Land: | Kérwá |
| Instelling: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/29507 |
| Online toegang: | http://www.sciencedirect.com/science/article/pii/S0041010103002964 https://hdl.handle.net/10669/29507 |
| Keyword: | Metalloproteinase Disintegrin Jararhagin Collagen Monoclonal Antibodies Hemorrhagic Activity Snake venom |