Venomics of the poorly studied hognosed pitvipers Porthidium arcosae and Porthidium volcanicum
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| Författarna: | , , , , , , , , , , |
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| Materialtyp: | artículo original |
| Utgivningstid: | 2021 |
| Beskrivning: | We report the first proteomics analyses of the venoms of two poorly studied snakes, the Manabi hognosed pitviper Porthidium arcosae endemic to the western coastal province of Manabí (Ecuador), and the Costa Rican hognosed pitviper P. volcanicum with distribution restricted to South Pacific Costa Rica and western Panamá. These venom proteomes share a conserved compositional pattern reported in four other congeneric species within the clade of South American Porthidium species, P. nasutum, P. lansbergii, P. ophryomegas, and P. porrasi. The paraspecific immunorecognition profile of antivenoms produced in Costa Rica (ICP polyvalent), Perú (Instituto Nacional de Salud) and Brazil (soro antibotrópico pentavalente, SAB, from Instituto Butantan) against the venom of P. arcosae was investigated through a third-generation antivenomics approach. The maximal venom-binding capacities of the investigated antivenoms were 97.1 mg, 21.8 mg, and 25.7 mg of P. arcosae venom proteins per gram of SAB, ICP, and INS-PERU antibody molecules, respectively, which translate into 28.4 mg, 13.1 mg, and 15.2 mg of total venom proteins bound per vial of SAB, ICP, and INS-PERU AV. The antivenomics results suggest that 21.8%, 7.8% and 6.1% of the SAB, ICP, and INS-PERU antibody molecules recognized P. arcosae venom toxins. The SAB antivenom neutralized P. arcosae venom's lethality in mice with an ED50 of 31.3 mgV/g SAB AV. This preclinical neutralization paraspecificity points to Brazilian SAB as a promising candidate for the treatment of envenomings by Ecuadorian P. arcosae. |
| Land: | Kérwá |
| Organisation: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Språk: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/85860 |
| Länkar: | https://hdl.handle.net/10669/85860 |
| Nyckelord: | Snake venomics Genus Porthidium Porthidium arcosae Porthidium volcanicum Antivenomics Latin American antivenoms Veneno de serpiente snake venom |