Proteoform-resolved quantitative venomics: looking through a crystal ball

 

Uloženo v:
Podrobná bibliografie
Autoři: Calvete Chornet, Juan José, Petras, Daniel, Calderón Celis, Francisco, Lomonte, Bruno, Ruiz Encinar, Jorge, Sanz Medel, Alfredo
Médium: artículo original
Datum vydání:2017
Popis:In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out comprehensive quantitative analysis of all toxins present in a venom. This short essay reflects
Země:Kérwá
Instituce:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/74787
On-line přístup:https://jvat.biomedcentral.com/articles/10.1186/s40409-017-0116-9
https://hdl.handle.net/10669/74787
Klíčové slovo:Top-down proteomics
Top-down venomics
Protein species resolved venomic
Absolute quantification
Inductively coupled plasma mass spectrometry
Snake venom