DNA barcoding the floras of biodiversity hotspots
محفوظ في:
| المؤلفون: | , , , , , , , , , |
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| التنسيق: | artículo original |
| تاريخ النشر: | 2008 |
| الوصف: | DNA barcoding is a technique in which species identification is performed by using DNA sequences from a small fragment of the genome, with the aim of contributing to a wide range of ecological and conservation studies in which traditional taxonomic identification is not practical. DNA barcoding is well established in animals, but there is not yet any universally accepted barcode forplants. Here, we undertook intensive field collections in two biodiversity hotspots (Mesoamerica and southern Africa). Using >1,600 samples, we compared eight potential barcodes. Going beyond previous plant studies, we assessed to what extent a ‘‘DNA barcoding gap’’ is present between intra- and interspecific variations, using multiple accessions per species. Given its adequate rate of variation, easy amplification, and alignment, we identified a portion of the plastid matK gene as a universal DNA barcode for flowering plants. Critically, we further demonstrate the applicability of DNA barcoding for biodiversity inventories. In addition, analyzing >1,000 species of Mesoamerican orchids, DNA barcoding with matK alone reveals cryptic species and proves useful in identifying species listed in Convention on International Trade ofEndangered Species (CITES) appendixes. |
| البلد: | Kérwá |
| المؤسسة: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| اللغة: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/90362 |
| الوصول للمادة أونلاين: | https://doi.org/10.1073/pnas.0709936105 https://hdl.handle.net/10669/90362 |
| كلمة مفتاحية: | CITES Kruger National Park Mesoamerica GENÉTICA VEGETAL |