Role of nitric oxide and signaling pathways modulating the stimulatory effect of snake venom secretory PLA2S on non-opsonized zymosan phagocytosis by macrophages
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| Autoři: | , , |
|---|---|
| Médium: | artículo original |
| Datum vydání: | 2024 |
| Popis: | The phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA2 homolog, and MT-III, an Asp-49 PLA2, from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT–III–induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or Rp-cGMPS (PKG inhibitor). These results indicate that the NO/sGC/GMP/PKG pathway plays an essential role in the β-glucan-mediated phagocytosis induced in macrophages by these venom-secretory PLA2s. |
| Země: | Kérwá |
| Instituce: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Jazyk: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104129 |
| On-line přístup: | https://www.sciencedirect.com/science/article/pii/S0041010124002885?via%3Dihub https://hdl.handle.net/10669/104129 https://doi.org/10.1016/j.toxicon.2024.107716 |
| Klíčové slovo: | nitric oxide phagocytosis snake venom PLA2s |