Citrate inhibition of snake venom proteases
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| Autores: | , , , , , , , |
|---|---|
| Fecha de Publicación: | 1998 |
| Cur Síos: | Thirty snake venoms had a citrate content of 2.3 to 12.9%, dry basis, by an aconitase–isocitric dehydrogenase coupled enzyme assay. This is a venom concentration range of approximately 30 to 150 mM citrate assuming 25% venom solids content. Inhibition of snake venom protease activity by the addition of exogenous citrate was obtained using azure blue hide powder and azocasein as substrates. Protease inhibitions of 7.5% for Crotalus atrox venom to 78% for Bothrops picadoi venom were observed with citrate. Complete inhibition of snake venom protease activity by citrate was not observed. Bothrops asper (Pacifico) venom showed a 41% protease inhibition by citrate with azocasein as the substrate and 46% inhibition of Bothrops asper (Alantico) venom protease with azure blue hide power as a substrate. Trypsin was not inhibited in this system. Citrate may inhibit some venom protease activity by forming a complex with the zinc of zinc-dependent enzymes. |
| País: | Kérwá |
| Institiúid: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Teanga: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104989 |
| Rochtain Ar Líne: | https://hdl.handle.net/10669/104989 https://doi.org/10.1016/S0041-0101(98)00084-1 |
| Palabra clave: | Snake Venom Crotalus atrox Bothrops asper Venom hemorrhagic toxins |