Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani

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書目詳細資料
Autores:	Arni, Raghuvir K., Fontes, Marcos Roberto de Mattos, Barberato, C., Gutiérrez, José María, Díaz Oreiro, Cecilia, Ward, Richard John
格式:	artículo original
Fecha de Publicación:	1999
實物特徵:	Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.
País:	Kérwá
機構:	Universidad de Costa Rica
Repositorio:	Kérwá
OAI Identifier:	oai:kerwa.ucr.ac.cr:10669/29449
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S0003986199912109 https://hdl.handle.net/10669/29449
Palabra clave:	Lys49-Phospholipase A2 Cerrophidion (Bothrops) godmani Myotoxins Snake venom