Coupling a bio-accumulator organism and MALDI-TOF MS: an early warning detection system for microcystins in water bodies
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| Autoři: | , , , , , , , , , , |
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| Médium: | artículo original |
| Datum vydání: | 2017 |
| Popis: | Microcystins produced by freshwater cyanobacteria pose serious threats to human health and are a growing problem in drinking water supplies worldwide. Toxin detection and identification in water reservoirs when cyanobacterial density is still low is a key step to predict harmful algal blooms and to allow the safe use of the resource. For this purpose, developing sensitive and rapid methods of analysis is crucial. Adopting a strategy that couples the use of a filter-feeder organism (Unio elongatulus, Pfeiffer 1825 (Bivalvia: Unionidae)) with matrix-assisted laser desorption ionization—time of flight mass spectrometry (MALDI-TOF MS) technology, effective microcystin detection was achieved. The performances of MALDI-TOF MS, high-liquid-performance chromatography with diode array detector (HPLC-DAD) and enzyme-linked immunosorbent assays (ELISA) test were compared. The results obtained in this study suggest that the exploitation of an efficient bio-accumulator organism, coupled with a rapid and sensitive analytical method, can be a very useful strategy in monitoring programs for early and prompt risk management. |
| Země: | Kérwá |
| Instituce: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Jazyk: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/83376 |
| On-line přístup: | https://link.springer.com/article/10.1007/s10811-017-1193-4 https://hdl.handle.net/10669/83376 |
| Klíčové slovo: | Microcystin detection Sentinel organism Unio elongatulus MALDI-TOF MS Early warning system |