Martin Chalfie y la proteína verde fluorescente
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| Materyal Türü: | artículo original |
| Durum: | Versión publicada |
| Yayın Tarihi: | 2012 |
| Diğer Bilgiler: | The history of the green fluorescent protein (GFP) starts with Osamu Shimomura at the beginning of the 1960s, with his discovery from the extracts of the Aequorea victoria jellyfish. However, it is not until the beginning of the 1990s, that the neurobiologist Martin Chalfie, applying genetic engineering tech- niques, achieved for the first time the heterology expression of the GFP in prokaryotes (E. coli) and eukaryotes (Caernohabditis elegans), from a clone of the jellyfish’s gen that codifies the GFP prepared by Douglas Prasher. Besides fluorescing in green, the results from this work also demonstrated that it does not require any other cofactor or enzyme of the jellyfish for its expression or for the own forming of a fluorophore. At the same time, Chalfie proposed that the GFP could be used for the marking of cells on living ani- mals or the tag of proteins. Based upon these results, many scientists started working on research related to the GFP; actually this protein is used on different fields such as biotechnol- ogy, development biology, environmental chemistry and medicine. In the case of the latter, it has helped to elucidate the cellular mechanisms from which diseases are made of. Science owes Roger Tsien, amongst other contributions, the creation of differ- ent mutations of the GFP. |
| Ülke: | Portal de Revistas TEC |
| Kurum: | Instituto Tecnológico de Costa Rica |
| Repositorio: | Portal de Revistas TEC |
| Dil: | Español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/624 |
| Online Erişim: | https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/624 |
| Anahtar Kelime: | GFP Aequorea victoria C. elegans expresión heteróloga PCR promotor fluoróforo. Caernohabditis elegans heterology expression promoter fluorophore. |