Raman spectroscopy analysis of DNA using the SERS method

 

Tallennettuna:
Bibliografiset tiedot
Tekijät: Varela-Fonseca, Stephanie, Montero-Zeledón, Ernesto, Rojas- Rojas, Laura, Varela-Fonseca, Alejandro, Gutiérrez-Fallas, Dionisio
Aineistotyyppi: artículo original
Tila:Versión publicada
Julkaisupäivä:2019
Kuvaus:The technological advances of the last decades have contributed to the development of new methodologies for the characterization of biological samples. Raman Spectroscopy has been used for the characterization of pure inorganic and organic compounds, but recently, it is being used to study molecular structures of biological importance. Fluorescence is usually present in Raman spectroscopy, shielding Raman signals, but using the SERS (Surface Enhanced Raman Spectroscopy) technique, it is possible to locally increase the Raman signal and to reduce the importance of the fluorescence effects on the samples. The SERS effect could be achieved by adding metallic nanoparticles, of a specific size distribution, to the sample. Usually gold and silver nanoparticles are used for SERS. In this research, DNA of human and Rickettsia bacteria were characterized by the SERS technique, with both silver and gold nanoparticles.
Maa:Portal de Revistas TEC
Organisaatio:Instituto Tecnológico de Costa Rica
Repositorio:Portal de Revistas TEC
Kieli:Español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/4501
Linkit:https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501
Sanahaku:Raman spectroscopy
SERS
nanoparticles
DNA
Espectroscopía Raman
nanopartículas
ADN