Human DNA Extraction by Two Extraction Methods for Forensic Typification from Human Feces on FTA Paper

 

Đã lưu trong:
Chi tiết về thư mục
Tác giả: Sandoval-Arias, Shirleny Monserrat
Định dạng: artículo original
Trạng thái:Versión publicada
Ngày xuất bản:2014
Miêu tả:The identification of suspects in criminal investigations has been facilitated since DNA test are executed on different samples. The application of this technology for forensic typification from human fecal samples still presents complications therefore this research evaluated two DNA extraction protocols with modifications to determine that of major efficiency. Organic extractions and extractions using the commercial kit “IQTM DNA Casework Sample Kit for Maxwell ® 16” on FTA portions of 4cm2 and 1cm2 impregnated with feces from the same individual were done to accomplish the objective. In all the assays the results were useful, however; the best forensic typification (by the electropherogram characteristics) was obtained by using the commercial kit in an area of 1 cm2 of FTA paper impregnated in a 1:4 dilution. 
Quốc gia:Portal de Revistas TEC
Tổ chức giáo dục:Instituto Tecnológico de Costa Rica
Repositorio:Portal de Revistas TEC
Ngôn ngữ:Español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/2081
Truy cập trực tuyến:https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/2081
Từ khóa:Forensic Human typification
human feces
DNA IQTM Casework Sample Kit for Maxwell ®16
organic extraction.
Tipificación forense
heces humanas
DNA IQTM Casework Sample Kit para Maxwell ®16
extracción orgánica.