Optimization of genetic transformation with Agrobacterium rhizogenes
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| Autors: | , , |
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| Format: | artículo original |
| Estat: | Versión publicada |
| Data de publicació: | 2003 |
| Descripció: | To optimize the genetic transformation efficiency using Agrobacterium rhizogenes, carrot sections inoculated with the Agrobacterium strain A4TC were co-cultivated with acetosyringone, phloroglucinol, and a mix of both. Acetosyringone is one of the phenolic compounds produced by plant tissues in response to wounding, which induces the transfer of T-DNA from the agrobacteria to the plant. Phloroglucinol is also a phenolic compound; however, it has a synergistic action with auxins by partially inhibiting cytokinin activity. The highest transformation efficiency (75%) was obtained with acetosyringone (100 mM) in combination with phloroglucinol (25 mg l-1). In general, a 6-day co-cultivation, independently of treatments, induced the best transformation rate. Inclusion of 100 mg l-1 kanamicin efficiently discriminated transformed roots from non-transgenic ones. This paper also presents a novel bacterial elimination method, by which Agrobacterium can be completely eliminated in 48 h with Cefotaxime at a dosage of 500 ml l-1. |
| Pais: | Portal de Revistas UCR |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/61655 |
| Accés en línia: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/61655 |
| Paraula clau: | Genetic transformation Agrobacterium rhizogenes acetosyringone phloroglucinol Transformación genética acetosiringona floroglucinol |