Purificación y separación de isoenzimas de L-aminoácido oxidasa del veneno de Bothrops asper

 

Gorde:
Xehetasun bibliografikoak
Egilea: Umaña, Virginia
Formatua: artículo original
Egoera:Versión publicada
Argitaratze data:1982
Deskribapena:L-amino acid oxidase (E.C. 1.4.3.2) was purified from the venom of Bothrops asper, snakes from Costa Rica. Three forms of this enzyme were obtained from the crude venom using only two steps: heating in the presence of L-leucine and chromatography on DEAE-cellulose. The isozymes were numbered 1, 2a and 2b on the basis of electrophoretic mobility towards the anode. Isozymes 1 and 2b were homogeneous by acrylamide gel electrophoresis and sedimentation velocity. They showed a molecular weight of 125,000 by sedimentation equilibrium.
Herria:Portal de Revistas UCR
Erakundea:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Hizkuntza:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/25343
Sarrera elektronikoa:https://revistas.ucr.ac.cr/index.php/rbt/article/view/25343