Environmental DNA exploration of fish diversity in the Balantak River, Central Sulawesi, Indonesia
Shranjeno v:
| Autores: | , , , , , , , , |
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| Format: | artículo original |
| Status: | Versión publicada |
| Fecha de Publicación: | 2026 |
| Opis: | Introduction: Freshwater ecosystems play a crucial role in supporting human life, providing many ecosystem services. Species diversity is a valuable indicator for evaluating ecosystem health, gaining a broader perspective on ecological richness and ecosystem functions, but data are lacking for many rivers. Objectives: To obtain baseline data on the upstream and downstream ichthyofauna of the Balantak River, Banggai Regency, using environmental DNA (eDNA) with a focus on diadromous species. Methods: We collected, filtered and preserved water samples from upstream and downstream sites on the Balantak River followed by eDNA analysis (DNA extraction, PCR with MiFish 12S rRNA primers, Illumina sequencing, MitoFish bioinformatic analysis at 97 and 99 % identity thresholds) to produce taxonomically assigned Actinopteri amplicon sequence variants (ASVs). Results: Our data are consonant with the expected dominance of diadromous and euryhaline taxa (75 %), high Gobiiformes biodiversity, and differences between upstream and downstream ichthyofaunal communities. Reads were assigned to Actinopteri ASVs from 12 orders, predominantly Gobiiformes, Anguilliformes, and Centrarchiformes. The 15 assigned families included Anguillidae, Eleotridae, Gobiidae, Rhyacichthydae, Kuhliidae, and Terapontidae. Of the assigned genera (17 at 97 % and 25 at 99 %), Anguilla had the most reads (39.9 %), and Sicyopterus had the most ASVs (8). Conclusions: These baseline data demonstrate the usefulness of eDNA metabarcoding for exploring ichthyofaunal biodiversity in small tropical watersheds while highlighting gaps in 12S rRNA sequence databases, reinforcing the need for integrative taxonomic research in Sulawesi watersheds and reliable reference databases. Most assigned taxa are vulnerable to exploitation for food and/or the ornamental fish trade. Four non-native taxa from Cichlidae, Cyprinidae and Clariidae are considered invasive. Implications for conservation include the need to address the unregulated and largely unreported freshwater ornamental trade and introduction of non-native invasive species. |
| País: | Portal de Revistas UCR |
| Institucija: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Jezik: | Inglés |
| OAI Identifier: | oai:portal.revistas.ucr.ac.cr:article/3754 |
| Online dostop: | https://revistas.ucr.ac.cr/index.php/rrbt/article/view/3754 |
| Ključna beseda: | Amphidromy Gobiiformes metabarcoding riverine biodiversity Wallacea Anfidromía metabarcodificación biodiversidad fluvial |