Immunohistochemical Analysis of Odontogenic Cystic Lesions: Diagnostic Concordance with CK14 and CK19 in a Prospective Study
Wedi'i Gadw mewn:
| Awduron: | , , , |
|---|---|
| Fformat: | artículo original |
| Statws: | Versión publicada |
| Dyddiad Cyhoeddi: | 2026 |
| Disgrifiad: | Accurate diagnosis of odontogenic cystic lesions remains challenging due to overlapping histopathological features. Immunohistochemistry (IHC) using cytokeratin markers may improve diagnostic precision. This study evaluated the diagnostic concordance between conventional histopathology and immunohistochemical analysis using CK14 and CK19 markers in odontogenic cystic lesions. A prospective single-center observational study was conducted analyzing 126 odontogenic cystic lesions from the Fundación Hospital Universitario Metropolitano between 2021-2023. All specimens underwent conventional histopathological examination followed by immunohistochemical staining with CK14 and CK19 antibodies. Diagnostic concordance was assessed using Cohen's kappa coefficient. The study included 126 cases with mean patient age of 42.3±16.8 years. Inflammatory cysts were most prevalent (35 cases, 27.8%). Overall diagnostic concordance between histopathology and immunohistochemistry was 72.2% (κ=0.68; 95% CI: 0.58-0.78; p<0.001). CK14 showed positive staining in 89.7% of cases, while CK19 was positive in 83.3%. Combined CK14+CK19 analysis improved diagnostic accuracy to 78.6% (κ=0.74; 95% CI: 0.65-0.83). Lesion size >3 cm (OR: 2.84; p=0.008) and extensive inflammation (OR: 3.21; p=0.003) were identified as independent predictors of diagnostic. Immunohistochemical analysis using CK14 and CK19 markers may provide valuable complementary diagnostic information in odontogenic cystic lesions, particularly in cases with extensive inflammation or large size. The combined use of both markers may enhance diagnostic precision, although multicenter validation is required before recommending standardized implementation. |
| Gwlad: | Portal de Revistas UCR |
| Sefydliad: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Iaith: | Inglés |
| OAI Identifier: | oai:portal.revistas.ucr.ac.cr:article/4989 |
| Mynediad Ar-lein: | https://revistas.ucr.ac.cr/index.php/rOdontos/article/view/4989 |
| Allweddair: | Odontogenic Cysts; Immunohistochemistry; Keratins; Diagnosis; Differential; Reproducibility of results; Jaw cysts. Quistes odontogénicos; Inmunohistoquímica; Queratinas; Diagnóstico diferencial; Reproducibilidad de resultados; Quistes maxilares. |