Susceptibility of the developmental stages L2 and L3 of Phyllophaga elenans to a native strain of Heterorhabditis sp. at greenhouse
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| Autores: | , , , |
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| Formato: | artículo original |
| Estado: | Versión publicada |
| Data de Publicação: | 2009 |
| Descrição: | The effect of 6 native entomopathogenic nematodes (EPN) strains over the 2nd instar of Phyllophaga elenans was evaluated. The nematode strain with the best results, Heterorhabditis sp. CIA-NE 07, was evaluated at 125, 225, 375, 500 y 625 nematodes.larva-1 to determine its pathogenicity over the 2nd and 3rd instar larvae of P. elenans. At the 7th day after inoculation, all doses except 125 nematodes.larvae-1 caused a significantly mortality of the 2nd instar, in comparison to the control, in which no deaths occurred. At the 14th, 21st, and 28th day all the inoculated treatments were significantly different from the control; the highest dosis caused 68% of mortality at day 30. It was determined that the median lethal doses corresponded to 475 nematode.larvae-1 and the median lethal time was 7.5, 8.5 y 17.5 days for the 625, 500 and 225 nematodes.larvae-1 concentrations, respectively. On day 7, EPN inoculation on third instar did not present significant differences among treatments; while at days 14, 21 and 28 only the 500 and 625 nematodes.larvae-1 doses presented a significantly mortality than the control. The highest mortality (24%), was obtained at day 30 with the 625 nematode.larvae-1 dose. The observed differences between second and third instars could be due to defense or escape mechanisms present in the 3rd instar of P. elenans. |
| País: | Portal de Revistas UCR |
| Recursos: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/6718 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/6718 |
| Palavra-chave: | Heterorhabditis Phyllophaga elenans nematodos entomopatógenos control biológico jobotos entomopathogenic nematodes biological control white grubs |