Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon
সংরক্ষণ করুন:
| লেখক: | , , |
|---|---|
| বিন্যাস: | artículo original |
| বর্তমান অবস্থা: | Versión publicada |
| প্রকাশনার তারিখ: | 1989 |
| বিবরন: | A lectin-like protein was isolated from L. muta venom by gel filtration on BIO Gel P-100 followed by column Chromatography on DEAE-Sephadex A-50. The protein eluted at 0.4 M NaO in 0.01 Tris pH 7.3 and exhibited agglutinin activity toward 0+ human erythrocytes. The protein is a dimer with Mr 28 kDa.Amino acid analysis revealed high content of tryptophan and acid residues and low content of cysteine and methionine residues. No neutral carbohydrates and sialic acid were detected. Circular dichroic spectrum shows 78% of B structure and 1% of α structure. In vitro experiments with ery throcytes from rat, rabbit and dog revealed strong agglutination while red blood cells from mice, sheep and goat were not agglutinated. In vivo experiments using non-anesthetized rats, a sharp and prolonged fall in the blood pressure was observed at protein dose of 1.5 mg/kg. Double dose of protein caused the death of the animal. |
| দেশ: | Portal de Revistas UCR |
| প্রতিষ্ঠান: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| ভাষা: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/23624 |
| অনলাইন ব্যবহার করুন: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/23624 |
| Access Level: | acceso abierto |