Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
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| Autori: | , , |
|---|---|
| Natura: | artículo original |
| Status: | Versión publicada |
| Data di pubblicazione: | 2006 |
| Descrizione: | Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. |
| Stato: | Portal de Revistas UCR |
| Istituzione: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Lingua: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/14108 |
| Accesso online: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108 |
| Access Level: | acceso abierto |
| Keyword: | clostridium tetani tetanus toxin enzyme immunoassay biological assay |