Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
Сохранить в:
| Авторы: | , , |
|---|---|
| Формат: | artículo original |
| Статус: | Versión publicada |
| Дата публикации: | 2006 |
| Описание: | Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. |
| Страна: | Portal de Revistas UCR |
| Институт: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Язык: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/14108 |
| Online-ссылка: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108 |
| Ключевое слово: | clostridium tetani tetanus toxin enzyme immunoassay biological assay |