Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays

 

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書目詳細資料
Autores: Chaves, Fernando, León, Guillermo, Hernández Chavarría, Francisco
格式: artículo original
狀態:Versión publicada
Fecha de Publicación:2006
實物特徵:Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
País:Portal de Revistas UCR
機構:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
語言:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/14108
在線閱讀:https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108
Palabra clave:clostridium tetani
tetanus toxin
enzyme immunoassay
biological assay