In vitro micropropagation of Lawsonia inermis(Lythraceae)
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| Authors: | , , , |
|---|---|
| Format: | artículo original |
| Status: | Versión publicada |
| Publication Date: | 2001 |
| Description: | A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3 % (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil. |
| Country: | Portal de Revistas UCR |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Language: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/18043 |
| Online Access: | https://archivo.revistas.ucr.ac.cr/index.php/rbt/article/view/18043 |
| Keyword: | in vitro micropropagation medicinal plants shoot multiplication |