A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats
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| Autors: | , , |
|---|---|
| Format: | artículo original |
| Estat: | Versión publicada |
| Data de publicació: | 2019 |
| Descripció: | Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. In animals, intraperitoneal administration of LPS, stimulates innate immunity and the production of pro-inflammatory cytokines. LPS provides an inflammatory stimulus that activates the neuroimmune and neuroendocrine systems resulting in a set of responses termed sickness behavior. The purpose of this protocol is to describe step-by-step the preparation and procedure of application of intraperitoneal injection of LPS in rats, as a guide for those researchers that want to use this assay to mount an inflammatory response. LPS intraperitoneal challenge in rats has been widely used to evaluate anti-inflammatory reagents and to address basic scientific questions. |
| Pais: | Portal de Revistas UCR |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/35510 |
| Accés en línia: | https://archivo.revistas.ucr.ac.cr/index.php/Odontos/article/view/35510 |
| Paraula clau: | Lipopolysaccharide; LPS challenge; LPS stimulation; Endotoxin; Inflammation |