Immuno-gold replica technique for ultrastructural localization of antigens
সংরক্ষণ করুন:
| লেখক: | , , |
|---|---|
| বিন্যাস: | artículo original |
| বর্তমান অবস্থা: | Versión publicada |
| প্রকাশনার তারিখ: | 1994 |
| বিবরন: | We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid. |
| দেশ: | Portal de Revistas UCR |
| প্রতিষ্ঠান: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| ভাষা: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/28717 |
| অনলাইন ব্যবহার করুন: | https://archivo.revistas.ucr.ac.cr/index.php/rbt/article/view/28717 |
| মুখ্য শব্দ: | Sendai virus immunolabelling immunoelectronmicroscopy replica ultrastructure |