Immuno-gold replica technique for ultrastructural localization of antigens
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| Autores: | , , |
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| פורמט: | artículo original |
| סטטוס: | Versión publicada |
| Fecha de Publicación: | 1994 |
| תיאור: | We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid. |
| País: | Portal de Revistas UCR |
| מוסד: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| שפה: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/28717 |
| גישה מקוונת: | https://archivo.revistas.ucr.ac.cr/index.php/rbt/article/view/28717 |
| מילת מפתח: | Sendai virus immunolabelling immunoelectronmicroscopy replica ultrastructure |