Immuno-gold replica technique for ultrastructural localization of antigens
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| Autores: | , , |
|---|---|
| Formato: | artículo original |
| Estado: | Versión publicada |
| Fecha de Publicación: | 1994 |
| Descrição: | We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid. |
| País: | Portal de Revistas UCR |
| Recursos: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/28717 |
| Acesso em linha: | https://archivo.revistas.ucr.ac.cr/index.php/rbt/article/view/28717 |
| Palavra-chave: | Sendai virus immunolabelling immunoelectronmicroscopy replica ultrastructure |